http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016096908-A1

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filingDate 2015-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6075917d73e55098c96c449c40ecc9c3
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publicationDate 2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2016096908-A1
titleOfInvention Biosensor based on single-molecule fluorescence detection
abstract A method for sensing an analyte [502] includes bringing a matrix containing the analyte into contact with a sensor device having a sensing volume containing fluorescent emitters [500] that switch from a first state [504] to a second state [506] when activated by chemical activation by the analyte. The fluorescent emitters are excited with optical radiation [510] to generate fluorescence optical radiation [514]. The excitation induces photo-bleaching of the activated fluorescent emitters, which limits the duration of the generated fluorescence optical radiation. Each resulting pulse of the fluorescence optical radiation is associated with a single one of the fluorescent emitters. The pulses are detected [516] and processed [518] to determine the presence/concentration of the analyte from changes in the detected fluorescence optical radiation.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11471077-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018158469-A1
priorityDate 2014-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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