| abstract |
The present disclosure relates to methods and systems of tissue engineering, and, more particularly, to optimization of tissue regeneration using cell cycle synchronization of stem cells. In an aspect, the disclosure provides for methods of tissue processing, comprising (a) synchronizing cell cycles of a plurality of cells, (b) wherein the synchronizing comprises suspending the cells in an agent; and (c) wherein the suspended cells are serum-starved. In another aspect, the cells comprise, consist of, or consist essentially of stem cells. In another aspect, the stem cells are mesenchymal stem cells (MSCs) or patient-specific pluripotent stem (iPS) cells. In yet another aspect, the cells comprise undifferentiated cells. |