| abstract |
The present invention relates to sequencing probes, methods, kits, and apparatuses that provide enzyme-free, amplification-free, and library-free nucleic acid sequencing that has long-read-lengths and with low error rate. A sequencing probe is used comprising a target binding domain and a barcode domain; wherein said target binding domain comprises at least four nucleotides and is capable of binding a target nucleic acid; wherein said barcode domain comprises a synthetic backbone, said barcode domain comprising at least a first attachment region, said first attachment region comprising a nucleic acid sequence capable of being bound by a first complementary nucleic acid molecule and wherein said nucleic acid sequence of said first attachment region determines the position and identity of a first nucleotide in said target nucleic acid that is bound by a first nucleotide of said target binding domain. |