Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2580cdb08c2c84de474963c93b779106 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate |
2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6eaf1fba978754e05d0ecbe3f9759c79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14fc83610ef02310b57cf6f52e159dd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a960b790bd7165a329624794c8761620 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21f123bd1a9a7e705734bc04980a5bf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0780a39d29a85096758398a8271c10f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c494befb513fca77f85c61a71ab02dc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f704199fa6ca3318e454cb916fb6960 |
publicationDate |
2016-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2016058127-A1 |
titleOfInvention |
Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent |
abstract |
Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor comprising a transposase identification sequence, and two ends of the broken nucleic acid are separately connected to the first adaptor and are separately provided with a gap; by means of purification or chemical reagent treatment, eliminating the influence of the transposase in the system on a follow-up reaction; connecting to a second adaptor at the gap by using a ligase, wherein a sequence of the second adaptor is different from a sequence of the first adaptor; and conducting a PCR reaction by using primers targeted to and combined with the first adaptor and the second adaptor respectively, so as to obtain a product whose two ends are respectively connected to different adaptor sequences. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11708573-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020510401-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021232184-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7164276-B2 |
priorityDate |
2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |