| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2580cdb08c2c84de474963c93b779106 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c494befb513fca77f85c61a71ab02dc3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21f123bd1a9a7e705734bc04980a5bf4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a960b790bd7165a329624794c8761620
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6eaf1fba978754e05d0ecbe3f9759c79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0780a39d29a85096758398a8271c10f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14fc83610ef02310b57cf6f52e159dd9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f704199fa6ca3318e454cb916fb6960 |
| publicationDate |
2017-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2016058126-A9 |
| titleOfInvention |
One-stop treatment method for breaking nucleic acid by means of transposase, and reagent |
| abstract |
Disclosed are a one-stop treatment method for breaking a nucleic acid by means of a transposase, and a reagent. The method of the present invention comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, the transposase-embedded complex comprising a transposase and a first adaptor comprising a transposase identification sequence; adding a first reagent to conduct treatment, so as to break an absorption effect of the transposase to a target sequence of the nucleic acid; adding a second reagent to conduct treatment, so as to weaken the influence of the first reagent on a follow-up enzyme-catalyzed reaction; and conducting a PCR reaction by using a product generated after the second reagent treatment as a template component, so as to obtain a PCR product of a broken nucleic acid segment whose two ends are connected to adaptors. |
| priorityDate |
2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |