| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d0f7c9219e444c5e2396d8f9720fc991
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e9d629d7ec53d93cdfc130442f95aad9 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbecd40c3e9e4d2544d71ccf8d1215f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33e607ccaaeb5465c459ccaff276051c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0486cef6457e7bea63a872d388595e43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8c6268d8f3972f91f6a463c68e11e1a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba2711116355bf7fd871ca74cbb2598e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77c9a9dd36d1e77f87ee5b1faff4c7e1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af0d802b8f26e7361e0da81237c2933b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_caaae9dc664e00fed3ffa661c22b3894
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09175657d80aed7206586a4ae2ae75bb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14b34c1cfc8d879e87cf00349e06a2e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1da9fc231dec5247d069aedec57d2e88 |
| publicationDate |
2016-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2016054870-A1 |
| titleOfInvention |
Method and use of nucleic acid isothermal amplification via polymerase spiral reaction |
| abstract |
Disclosed are a method and use of nucleic acid isothermal amplification via a polymerase spiral reaction. Also provided is a primer comprising a specific primer and n accelerating primers A; the specific primer consists of a forward primer FP and a backward primer BP; the forward primer FP sequentially consists of an N region and an F region from a 5' end to a 3' end, the F region being identical to or complementary with an Fc region adjacent to the 3' end in a target sequence; the backward primer BP sequentially consists of an N' region and a B region from a 5' end to a 3' end, the B region being identical to or complementary with a Bc region adjacent to the 5' end in a target sequence; and the N' region is a reverse non-complementary sequence of the N region. The nucleic acid isothermal amplification method of the present invention completes nucleic acid amplification with only one pair of primers, employs a self-spiraling amplification method, and has a high amplification efficiency. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110628950-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110628950-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10544452-B2 |
| priorityDate |
2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |