http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016048046-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-942 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-865 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2015-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17928caed0d878c44f1177cd91a89580 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17b3f5fd09f0841dba6d11f909e00fa8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1311c54ab87d52046fd02dc97e4539a6 |
| publicationDate | 2016-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2016048046-A1 |
| titleOfInvention | Method for increasing mannose phosphorylation by using ylmpo1 overexpression |
| abstract | The present invention relates to a method for increasing mannose phosphorylation by using YlMpo1 overexpression and, more specifically, to a method for preparing a bi-mannosylphosphorylated glycan-added protein by overexpressing YlMpo1 proteins in a recombinant yeast in which a yeast-specific glycan biosynthetic pathway is removed. The bi-mannosylphosphorylated glycan, prepared by the method of the present invention, is easily converted into a bi-phosphorylated glycan to which two mannose-6-phosphates are added by an enzymatic or chemical method, and then a trimming step is performed by a mannosidase treatment, so as to be converted into a glycan (P 2 -Man 6 GlcNAc 2 ) having the structure, which has high binding affinity for a mannose-6-phosphate receptor and has a superior lysosomal targeting ability. Therefore, the present invention can overexpress the YlMpo1 in a yeast, in which a yeast-specific glycan biosynthetic pathway is removed, so as to produce a glycoprotein to which a high content of the bi-mannosylphosphorylated glycan is added, thereby providing a glycoprotein having a superior lysosomal targeting ability. |
| priorityDate | 2014-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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