| abstract |
A method for synthesizing cis, cis -muconic acid from a low value carbon source, comprising the steps of oxidizing the low value carbon source with at least one oxidizing agent to produce a modified, partially solubilized low value carbon source; and contacting the modified, partially solubilized low value carbon source with a genetically engineered microorganism adapted for growth in the modified, partially solubilized low value carbon source, which is derived from a wild-type microorganism comprising a cis, cis -muconic acid biosynthesis pathway from protocatechuate. A genetically engineered microorganism selected from the genus of Rhodococcus is also provided. |