Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8386f8f06597f6eac0c1a13ba61a214f |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-1866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5176 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5184 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5192 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
filingDate |
2015-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2c27b7eee03ae947e687cc0fb7bc9df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d4f146d112ea447a3da0fd4c228b86a |
publicationDate |
2016-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2016033695-A1 |
titleOfInvention |
Exosome isolation |
abstract |
A method of isolating exosomes from a biological sample is provided. The method includes the steps of: i) exposing the biological sample to a first centrifugation to remove cellular debris greater than about 7-10 microns in size from the sample and obtaining the supernatant following centrifugation; ii) subjecting the supernatant from step i) to centrifugation to remove microvesicles therefrom; iii) microfiltering the supernatant from step ii) and collecting the microfiltered supernatant; iv) subjecting the microfiltered supernatant from step iii) to at least one round of ultracentrifugation to obtain an exosome pellet; and v) re-suspending the exosome pellet from step iv) in a physiological solution and conducting a second ultracentrifugation in a density gradient and remove the exosome pellet fraction therefrom. The method advantageously results in exosomes that maintain integrity, stability and are essentially free from contaminating particles. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019527050-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11684893-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951669-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11477981-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019022542-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10874610-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018075825-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019520408-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022182782-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2651521-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105802912-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022164688-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019077534-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018187686-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10955410-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384743-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107702957-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2741776-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7171965-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11298319-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111019885-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111019885-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018107061-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3648777-A4 |
priorityDate |
2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |