http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016029889-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_99ffc11605daa6969b687ae827350e71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7307b233757226bbd4ecfb3fceee4f4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de8acc4d2befaea893b46e36ae46692a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-978 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 2015-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd2539f947a9b1c60f0060dff3af501c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d99c8e6057b747b04e91505a0d838d8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0904713947cc2d0a7db11d3369af979 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32d8ff732f573ca75b8253fb16f54c3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba67903213a2e906d3557b3ac8305950 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e4579fc7845886c2439b129902055b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_664ed64e0729d4206f00dad2b6c0671b |
| publicationDate | 2016-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2016029889-A1 |
| titleOfInvention | Method of determining the activity of enzymes converting cytosine derivatives to uracil derivatives in cells, tissues and organisms |
| abstract | The invention provides a method of determining the deaminase activity of the enzymes transforming cytosine derivatives into uracil derivatives in a sample, wherein the sample is incubated with a substrate of an enzyme selected from a group comprising 5-ethynyl-2'- deoxycytidine (EdC), 5-bromo-2'-deoxycytidine (BrdC), 5-chloro-2'-deoxycytidine (CldC), 5-iodo-2'-deoxycytidine (IdC), 5-ethynylcytidine (EC), 5-bromocytidine (BrC), 5- chlorocytidine (CIC), 5-iodocytidine (IC) and 5-fluorocytidine (FC), whereas the substrates in the presence of enzymes transforming cytosine derivatives into uracil derivatives are subsequently transformed in the sample into deaminated products, EdC is transformed into 5-ethynyl-2'-deoxyuridine (EdU), BrdC into 5-bromo-2'-deoxyuridine (BrdU), CldC into 5-chloro-2'-deoxyuridine (CldU), IdC into 5-iodo-2'-deoxyuridine (IdU), EC into 5- ethynyluridine (EU), BrC into 5-bromouridine (BrU), CIC into 5-chlorouridine (CIU), IC into 5-iodouridine (IU) and FC into 5-fluorouridine (FU), these deaminated products are subsequently detected in the DNA or RNA and the amount of incorporated EdU, BrdU, CldU, or IdU in the DNA, and EU, BrU, CIU, IU, or FU in the RNA are determined. The invention also includes the use of the above-mentioned method for determining the deaminase activity in cells treated with substances, which influence the deaminase activity of the enzymes transforming cytosine derivatives into uracil derivatives. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109207535-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7314289-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109207535-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022519116-A |
| priorityDate | 2014-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 354.