| abstract |
A coryneform bacterial transformant that has been subjected to the following manipulations (A), (B), (C), and (D) can efficiently produce shikimic acid using a saccharide as a raw material. (A) Enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) loss, inhibition, or reduction of sugar uptake into cells via phosphotransferase system (PTS); (C) enhancement of glucokinase activity and activity for taking up sugar into the cells via a sugar transporter different from the phosphotransferase system; (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity |