| abstract |
The present invention addresses the problem of providing a method for producing a lipid particle, said lipid particle being capable of holding a nucleic acid, etc. at a high encapsulation rate, and a nucleic acid delivery carrier comprising the lipid particle. Provided is a method for producing a lipid particle having a nucleic acid encapsulated therein, said method comprising:n(1) a step for heating an oil phase comprising a phospholipid, an alcohol and an ester;n(2) a step for mixing an aqueous phase containing a nucleic acid with the oil phase prepared in step (1);n(3) a step for cooling the liquid mixture obtained in step (2), said liquid mixture comprising the oil phase and the aqueous phase, and crystallizing lipid particles; andn(4) a step for removing the alcohol and ester from the liquid mixture obtained in step (3), said liquid mixture comprising the oil phase and the aqueous phase. |