| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_93f1ee19dc8b1585c20bc59c0f68ed70 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2015-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ce485f1888097bdbc49f8e0713ca22d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df85ad6aa613207fa5f7d71500a45eff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49213a6b05e96ec9ad94ba79bf4edef8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da3fc0aa9a406b107531b545470b62b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e81a3ccc9b7ea1a94062c6ca775b7032 |
| publicationDate |
2015-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2015172080-A1 |
| titleOfInvention |
Integrated single cell sequencing |
| abstract |
This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017204294-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11701392-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2017204294-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017092204-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3917660-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11248228-B2 |
| priorityDate |
2014-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |