| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c72d118f5664072de841f9c5c34b9d99 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2015-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3ba42a1ca9d788ac92cace69190788e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_748540162a3e7edd75199ea3d714e433
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf8fec3bff66b856ef30a890ca45294c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_959959d7efc44fe64b5b29f570938c06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d03b95cf6400aa02a4ba88edc00e245b |
| publicationDate |
2015-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2015148218-A2 |
| titleOfInvention |
Isothermal amplification under low salt condition |
| abstract |
Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5' end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10 C below the reaction temperature. The amplification is effected under isothermal condition. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7167024-B2 |
| priorityDate |
2014-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |