Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37b416a8c0d2b0f1c3d6026d932027af |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-603 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-602 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-11 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate |
2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8a2ed8bb0e1c6242e2b44e45614f857 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2dc56d561a9c5a5d15dd50ee9ad93a2 |
publicationDate |
2015-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2015137885-A1 |
titleOfInvention |
Method for inducing pluripotency in a hematopoietic cell |
abstract |
There is provided a method of inducing pluripotency in a hematopoietic cell. The method comprises providing a blood sample that has been collected from a subject in the absence of any polysaccharide preparation used for separating blood components. The sample is depleted of red blood cells by treating the sample with a hypotonic erythrocyte lysis buffer, in the absence of any polysaccharide preparation used for separating blood components, thus obtaining a remaining cell population. The remaining cell population in the sample is expanded in a hematopoietic expansion medium to obtain an expanded cell population that contains CD71+ cells, and the expanded cell population containing the CD71+ cells is then cultured in the presence of Oct4, Sox2, and Klf4, and optionally c-MYC, in human embryonic stem cell medium to induce pluripotency. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113481290-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113481290-B |
priorityDate |
2014-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |