| abstract |
The present invention relates to: a method for preparing a target recombinant protein of which the galactosylation is controlled or a method for controlling the galactosylation of a target recombinant protein, comprising a step of increasing the osmotic pressure of a culture solution of animal cells, which express a target recombinant protein, during the culturing of the animal cells; a method for preparing a target recombinant protein of which the galactosylation is controlled or a method for controlling the galactosylation of a target recombinant protein, comprising a step of supplementing asparagine to a culture solution of animal cells, which express a target recombinant protein, during the culturing of the animal cells; a method for preparing a target recombinant protein of which the galactosylation is controlled or a method for controlling the galactosylation of a target recombinant protein, comprising a step of increasing the osmotic pressure of a culture solution of animal cells, which express a target recombinant protein, and supplementing asparagine thereto during the culturing of the animal cells; and a target recombinant protein of which the galactosylation is controlled, which is prepared by the method. |