http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015112099-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f0601d923869481b0d51082e7f620581 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-44 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-44 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-20 |
filingDate | 2014-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_318412720ee8bfef019247e3af4d5ea9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a2c7760a57e5c23df1fcedb27be7a6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a28d234b37b68bfd78023db97a95c095 |
publicationDate | 2015-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2015112099-A1 |
titleOfInvention | Production of immunoreactive proteins by using the methods of recombinant dna technology that usable for serological diagnosis of babesia ovis infection |
abstract | Invention relates to the production of immunoreactive proteins obtained from Babesia ovis organisms by using the methods of recombinant DNA technology. The specific genes encoding these proteins were detected with immunoscreening of cDNA library constructed from mRNA of Babesia ovis. After cloning of these specific genes in the pGEX expression vectors, recombinant vectors were transformed to the competent E. coli cells for expression of recombinant proteins. The proteins were expressed as GST-fusion proteins in E. coli cells with IPTG induction, and were purified by glutathione affinity chromatography. The results of ELISA using these GST-fusion proteins as antigens showed that the two immunoreactive proteins detected B. ovis-specific antibodies in the eighth day of experimental infection. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108315341-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110082534-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018372742-A1 |
priorityDate | 2014-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.