| abstract |
Provided is a compound represented by formula I, wherein R 9 is a C1-C22 hydrocarbyl group or a C2-C3 ester-substituted C1-C3 alkyl group, R 5 , R 6 , R 7 and R 8 are alkyl groups or H, and R 1 , R 2 , R 3 and R 4 are H or lower hydrocarbyl groups. Or, R 9 is a C2-C22 hydrocarbyl group or a C2-C3 ester-substituted C1-C3 alkyl group, and R 5 is connected to R 1 , R 6 is connected to R 2 , R 7 is connected to R 3 and R 8 is connected to R 4 to form six-membered rings. The present compound is temperature-sensitive and can enter into cells. An intracellular temperature distribution image having high spatial and temporal resolution can thus be obtained. The present compound can also perform distribution calibration on a temperature-sensitive fluorescent compound. Also provided is a method for measuring temperature distribution within a living cell, and a corresponding detection kit. The method satisfies the requirements of small size measurement and rapid measurement, thereby achieving high resolution in terms of space and time. |