http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015098663-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2440-38 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
filingDate | 2014-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41bce6fbcdc57a7f30a5c5c146a2273f |
publicationDate | 2015-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2015098663-A1 |
titleOfInvention | Method for determining site having n-linked sugar chain added thereto or proportion of said addition |
abstract | Provided are: a method for detecting a site which can be modified with an N-linked sugar chain and to which an N-linked sugar chain is actually added in a sugar protein; and a method for determining the state of addition of an N-linked sugar chain at the site. A sugar protein having an N-linked sugar chain added thereto is subjected to an N-linked sugar chain removal treatment with a peptide N-glycanase, subsequently a site capable of being modified with an N-linked sugar chain, in which an Asn residue has been mutated to an Asp residue by the action of the peptide N-glycanase, is treated with an endo-type peptidase capable of recognizing an Asp residue to thereby produce peptide fragments, and subsequently the mass of the fragments is detected. In this manner, a site which can be modified with an N-linked sugar chain and to which an N-linked sugar chain is actually added can be detected. Furthermore, the proportion or state of the addition of the N-linked sugar chain at the site can be determined from the intensity of a signal generated upon the detection. |
priorityDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 129.