| abstract |
The invention refers to a process of mixotrophic cultivation of unicellular algae. Through this process the compounds resulted from preparation of algal biomass processed for biofuels production are used for supplementation of the autotrophic cultivation media, respectively raw glycerol, resulting from the transesterification of algal lipids, as source of carbon, and hydrolysates of proteins and nucleic acids from unicellular algae defatted biomass, as source of nitrogen and phosphorus. Raw glycerol is added in concentrations between 2 and 5 g/l mixotrophic media and hydrolysates of proteins and nucleic acids from algal biomass, normalized to 5% toal nitrogen and 1.5% total phosphorus, are used in the concentration of 12.5 g/l. |