| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_faf522b8b83eded745c73018576219ba |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91091
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91097
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91215
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90209 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-008 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
| filingDate |
2014-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3294b9cf05206033672b338ac6b0adf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9c1c36fd47bde18be092923e4e5d59b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef174f7e1a43ee94afd0ac1798b4cc23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a61d320cf83448e639fcb489ba424ae5 |
| publicationDate |
2015-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2015076361-A1 |
| titleOfInvention |
Method for detecting fluorescence or absorbance, method for suppressing background, method for measuring adp, method for measuring activity of adp-synthesizing enzyme, and method for measuring activity of glucosyltransferase |
| abstract |
A method for detecting fluorescence or absorbance according to the present invention is characterized by comprising reducing resazurin into resorufin by a diaphorase in the presence of an SH reagent and NADH or NADPH and then measuring the resultant fluorescence intensity or absorbance. A method for measuring ADP according to the present invention is characterized by comprising: step (2-1) for treating glucose with ADP and ADP-dependent hexokinase; step (2-2) for treating glucose-6-phosphate obtained in the above step (2-1) with NAD or NADP and glucose-6-phosphate dehydrogenase; and step (2-3) for treating resazurin with NADH or NADPH obtained in the above step (2-2) and a diaphorase in the presence of an SH reagent and then measuring the resultant fluorescence intensity or absorbance. |
| priorityDate |
2013-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |