http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015073744-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_58efc499451bfaca6cf77e3a8bd454cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a3c695cce0518f30843fb9efb08fde2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d3dca9501b06a2eb22c2c15d18538bba http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f1ce4f402165475221101f3d4ccc4705 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ccd86204e23273440df8a756cf47d9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed0be8ca59f97cb4d9c481197c02a691 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10f49e5a61d50e831ee335dfbbe1259f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b4cc793bca44ed584e0cd7b53187cb |
| publicationDate | 2015-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2015073744-A1 |
| titleOfInvention | Ultrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics |
| abstract | This disclosure employs the combination of a microfluidics platform and drop-based digital polymerase chain reaction (dPCR) to create a breakthrough technology that enables the detection of CTC. genes and the isolation of single CTCs from the blood. In the first method, cDNA molecules from lysed CTCs are amplified in microfluidic drops and detected via fluorescence signal. In the second method, intact single CTCs are encapsulated, and amplification-positive drops are sorted from the remaining cells. To demonstrate the clinical utility of our technology, mutations in the KRAS gene in colorectal cancer are analyzed to study resistance to EGFR-based treatment as a test case. The methods herein present robust techniques for both the diagnosis and treatment of cancers, as well as for the obtainment of a pure CTC sample from billions of other cells in the blood. |
| priorityDate | 2013-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.