| abstract |
The invention relates to a method for labeling specifically living bacteria of a given category of Gram negative bacteria in a sample comprising bacteria, the method comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to react with a second reactive group, so that a monosaccharide residue bearing said first reactive group is incorporated into the polysaccharides of the outer membrane of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the outer membrane of the bacteria, with a labeling molecule comprising a said second reactive group. The said modified monosaccharide compound is a compound having the following formula (I), or a salt thereof: Wherein X can be O, NH or S, R1, R2 and R3 can be independently H, OH, NH 2 , OH and NH 2 being substituted or not by protecting groups thereof, and R4 is H or an alkyl chain in C 1 to C 4 each carbon being substituted or not by OH or NH 2 substituted or not by protecting groups thereof and at least one of X, R1, R2, R3 and R4 groups being substituted by a said first reactive group R'1. |