| abstract |
The application relates to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples. In one embodiment the method includes a step of depleting single-stranded nucleic acids from the sample. In another embodiment the method includes a step of hybidising random primers to single-stranded DNA obtained from the sample. In a further embodiment the method includes a step of selectively shearing nucleic acids obtained from the sample. |