| abstract |
The present disclosure generally relates to the field of nanopore-based analysis of polymers, such as RNA and DNA polymers. In one aspect, the disclosure provides methods of analyzing a nucleic acid in a nanopore-based system that include application of nonconstant electrical potentials as the nucleic acid translocates through the nanopore. The methods facilitate the realization of informative continuous current profiles to ascertain characteristics of the nucleic acid analyte. In another aspect, the disclosure provides systems and methods for reducing the Brownian motion of nucleic acid analytes in nanopores to provide for enhanced resolution of nanopore-based analyses. |