| abstract |
The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R 1 -CO-O-[C m H n O x ] y -O-R 2 , wherein R 1 is a saturated or non-saturated C 1 -C 30 alkyl, R 2 is a sterol moiety, m = 5-7, n = 9-14 and x = 5-7, y = 1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat. |