http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014198964-A2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c86be5ec48bb387106aee05b221a93f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_192cb44d1b57f11acff18ee2de912b9e |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01001 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06 |
filingDate | 2014-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99d04703526c33663c4574c1dd74f24c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69c70e21ec938522bb57f897717f00e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a99a72e1cf27a0d9a69b9dcd5231e057 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_886df12e4665505c5c872be1c037be85 |
publicationDate | 2014-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2014198964-A2 |
titleOfInvention | Metabolically enhanced cyanobacterial cell for the production of ethanol |
abstract | A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn 2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The alcohol dehydrogenase enzyme has (i) a Michaelis constant K m for NADPH which is lower than the Michaelis constant K m for NADH of the alcohol dehydrogenase enzyme, and (ii) a Michaelis constant Km for acetaldehyde which is lower than 0.08 ⋅10 -3 M. Alternatively, the alcohol dehydrogenase enzyme has a Michaelis constant K m for acetaldehyde which is higher than 0.65 ⋅10 -3 M but lower than 10 ⋅10 -3 M in combination with a Michaelis constant K m for ethanol which is higher than 20 ⋅10 -3 M. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9493795-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9493794-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9127297-B2 |
priorityDate | 2013-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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