| abstract |
The present invention discloses improved formulation of probiotic cultures of lactic acid bacteria with apigenin (2) and peppermint (Mentha piperita L.) extract, process of its preparation, and use thereof. The formulation comprising: (i) one or more lyophilized cultures of probiotic bacteria selected from the groups consisting of: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus bulgaricus, Lactobacillus johnsonii, Lactobacillus delbrueckii, Bifidobacterium animalis ssp. lactis and Bifidobacterium longum;- in -amount from 1»10 7 to 5·10 10 cfu/g or 0.01-50% w/w; (ii) apigenin (1), its O-glycosides, or dry herbal extract containing them, in amount from 10-50% w/w calculated to apigenin (1) content; (iii) dry extract of peppermint (Mentha piperita L.) leaf; in amount from 10-50% w/w and (iv) excipients required to- give final dosage forms to 100%- w/w cf the formulation. The formulation- is used as food, pharmaceutical: or veterinary product. |