| abstract |
Methods for modifying the genome of plants at a target nucleic acid sequence are provided. Such methods involve producing a plant cell comprising a Cas protein and a single guide RNA (sgRNA) that is capable of recognizing the target nucleic acid sequence in the plant cell. Further provided are methods for targeting fusion proteins to target nucleic acid sequences in the genome of plant. Such methods involve producing a plant cell comprising a fusion protein and an sgRNA designed to recognize the target nucleic acid sequence. The fusion protein is capable of recognizing the target sequence in the presence of the sgRNA. The fusion protein comprises a Cas protein domain that is defective in nuclease activity and an additional domain. Also provided are methods for testing components of the Cas system in plants, modified plants and plant cells, fusion proteins, and nucleic acid molecules encoding such fusion proteins. |