| abstract |
The invention relates to methods of preparing and assessing purified preparations of an RNA comprising a modified nucleoside. Thus, in one embodiment, the invention is a purified preparation of messenger RNA comprising a 1-methylpseudouridine residue. In some embodiments, the messenger RNA also comprises a polyA tail. In some embodiments, the messenger RNA also comprises an m7GpppG cap. In some embodiments, the messenger RNA also comprises a cap-independent translational enhancer. In some embodiments, the messenger RNA comprises at least about 95% to about 99.9% of all the nucleic acid present in the purified preparation. In some embodiments, the purified preparation of messenger RNA is significantly less immunogenic than an unpurified preparation of messenger RNA with the same sequence. |