abstract |
The present invention relates to a method for producing D-chiro inositol from myo-inositol using resting cells of recombinant cells expressing myo-inositol transporter, inositol dehydrogenase and inosose isomerase. The method according to the present invention uses minimal medium, can use resting cells repeatedly, and produces equivalent yields for myo-inositol to D-chiro inositol coversion when compared with using complete medium, thus production costs of mass producing D-chiro inositol can be significantly reduced. Furthermore, as minimal medium is used for cultivation, compared with using complete medium, the efficiency of purifying D-chiro inositol can be significantly improved. |