http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014097321-A1

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filingDate 2013-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb8a1c38b963eec67cca6ce46b5dc8b6
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publicationDate 2014-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2014097321-A1
titleOfInvention Development of bioprocess for production of novel cutinases from thermobifida fusca in e. coll bl21 (de3) [accession no. mtcc 5725 and accession no. mtcc 5726]
abstract The invention relates to a process of production of novel cutinases from Thermobifida n fusca NRRL B-8184 strain in E. coli BL21 (DE3) using pET22b (+) expression vector. It specifically relates to recombinant construction, expression and production of two cutinase enzymes, Cut_l and Cut_2 of Thermobifida fusca NRRL B-8184 in genetically engineered E. coli BL21 (DE3) separately [Accession No. MTCC 5725 and Accession No. MTCC 5726]. Physiochemical parameters for the production were optimized and five different production mediums were screened for higher level production of recombinant cutinase. Under optimal conditions, the production of recombinant Cut_l and Cut_2 (cutinases) in one of the production medium was found to be 11 fold higher than the wild type or known process.
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