http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014097321-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9521b8feb080b7a9dd2c47c2e51fe7d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f8c8a04ff399fe92bcf9a96638fa395 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
filingDate | 2013-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb8a1c38b963eec67cca6ce46b5dc8b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_203a87e6caea5915a0521f1d1741f871 |
publicationDate | 2014-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2014097321-A1 |
titleOfInvention | Development of bioprocess for production of novel cutinases from thermobifida fusca in e. coll bl21 (de3) [accession no. mtcc 5725 and accession no. mtcc 5726] |
abstract | The invention relates to a process of production of novel cutinases from Thermobifida n fusca NRRL B-8184 strain in E. coli BL21 (DE3) using pET22b (+) expression vector. It specifically relates to recombinant construction, expression and production of two cutinase enzymes, Cut_l and Cut_2 of Thermobifida fusca NRRL B-8184 in genetically engineered E. coli BL21 (DE3) separately [Accession No. MTCC 5725 and Accession No. MTCC 5726]. Physiochemical parameters for the production were optimized and five different production mediums were screened for higher level production of recombinant cutinase. Under optimal conditions, the production of recombinant Cut_l and Cut_2 (cutinases) in one of the production medium was found to be 11 fold higher than the wild type or known process. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108946929-A |
priorityDate | 2012-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.