abstract |
A method in which, using a microorganism and/or a culture thereof, an enzymatic reaction system using acetoacetyl-CoA reductase and enoyl-CoA hydratase is used to manufacture 1,4-butanediol by way of acetoacetyl-CoA, 3-hydroxybutyryl-CoA, and crotonyl-CoA, in that order. Said acetoacetyl-CoA reductase and enoyl-CoA hydratase are each specific to a stereoisomer of 3-hydroxybutyryl-CoA. |