http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014064710-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3a0d6297082cc4413878148c761e49b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_39fcd26047abd678aa22b393d9a42215 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2013-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f425c9abbd859a5f2b00cb766b0c09c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e9cb2a3a07dbb8dd289e5e54ff0d99c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2caff9466c4ab4292b89ef5c7b4ac403 |
| publicationDate | 2014-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2014064710-A1 |
| titleOfInvention | A process for the prepartion of non-viral vector for delivery of nucleic acids by mucosal route |
| abstract | The invention relates to a process for preparation of non-viral vector for delivery of nucleic acids by mucosal route comprising the steps' of (a) synthesis of pDNA loaded calcium phosphate nanoparticles using reverse micro-emulsion method having n- Hexane as oil phase and water as the aqueous phase; (b) addition of excess water to make total volume of water to adjust the molar ratio of water to AOT at 10 before stirriung both the micro-emulsions; (c) taking 1.36M of anhydrous calcium chloride and 0.35 M di-sodium hydrogen phosphate in two separate micro-emulsion system as the precursors; (d) adding 3μg of pDNA of interested into each system followed by continuous stirring for 12 hours; (e) mixing the micro- emulsion with di-sodium hydrogen phosphate to the micro-emulsion with calcium chloride at slow rate with continuous stirring at 4°C; (f) keeping the mixture, as obtained in step (e), undisturbed at low temperature under continuous stirring for 24 hours; (g) removing n- Hexane by using Bucci-evaporator and dissolving the resulting mass of AOT in 10 ml of absolute ethanol (99.9%) by vortexing; (h) centrifuging the solution for half an hour at 800 rpm at 4°C in a cold centrifuge; (i) washing the pelleted nanoparticles with absolute alcohol three times; (j) dispersing the pelleted nanoparticles in double distilled water at 4°C by vortexing to secure clear dispersion; and (k) dialyzing the dispersion, as obtained in step (j), in cold room using 14 kD dialysis membrane bag and followed finally for coating over nanoparticles with the polymers. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114522137-A |
| priorityDate | 2012-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.