| abstract |
The disclosure features methods and systems (100) that include a detector (112) configured to obtain multiple images of a sample (108) stained with first and second stains, preferably CellMask™ Blue and Hoechst/DAPI, where the first and second stains have similar spectral absorption and emission profiles, and an electronic processor (116) configured to decompose the multiple images into an unmixed image set, where the unmixed image set includes a first unmixed image corresponding to the first stain and a second unmixed image corresponding to the second stain, and identify nuclear regions in the sample (108) based on the first unmixed image and identify cytoplasm regions in the sample (108) based on the second unmixed image. |