http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014029894-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fe3a42ddd0f5252a9fb0d7130419116f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b015e832948029f79f728d85a7ed2326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_257701823e2206179fdddcff45764006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aed5b073b928cd37b98d26428d8b2c2 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2012-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2257388c6feba25e94ce7c18489287cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1dacc01f9242b77c43fbbbc7d2019e82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_090ef19f6277572af025fc128e410d65 |
| publicationDate | 2014-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2014029894-A1 |
| titleOfInvention | Method for separating tagged polypeptides |
| abstract | The present invention relates to a method for separating polypeptides or proteins tagged with histidine glues from a mixture of proteins (for example, a cell extract), by means of a system of two aqueous phases in which one of the components is PEG, and in which the protein tagged with polyhistidine is preferably distributed in the PEG-rich phase under specific conditions. The purification process can include one or more washing steps and is completed with an inversion in the distribution of the tagged polypeptide or protein, induced by a change in the pH or by the addition of choline, which leads to the recovery of said peptide in the opposite phase. The described method is simple, economically efficient, scalable and does not require heavy-metal ions; said method is therefore suitable for use in the industrial sector. |
| priorityDate | 2012-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.