| abstract |
The present invention provides a dicotyledon transgenic method for invading growing points of seed sprouts or seedling stems minimally and fully, comprising: removing a cotyledon to expose growing points, and pricking and brushing the growing points by using a minimally-invasive brush of which the diameter of a single brush hair is 4 to 20 micrometers, the length of the brush hair is 0.5 to 3 millimeters and the number of the brush hairs is 100 to 5000 and which is dipped with agrobacterium mediated conversion liquid; and performing identification on T 1 generation. The present invention is applicable to all seed bearing dicotyledons. A conversion effect that the conversion rate of cotton is 50%, and the conversion rate of soybeans is 76.5% during the conversion of the cotton and the soybeans is achieved. |