| abstract |
Some aspects of this disclosure provide a method for separating a glycan and/or a polypeptide fraction from a mixture comprising a glycan and a polypeptide. In some embodiments, the method comprises acidifying the mixture in order to decrease non-covalent glycan/polypeptide interactions prior to separation. Reagents and devices useful for glycan and/or polypeptide separation are also provided. The technology disclosed herein allows for the rapid, cost-efficient separation and isolation of both glycans and polypeptides obtained from glycosylated proteins after release of the glycans, for example, by enzymatic digest. Uses of the disclosed technology include, for example, the separation and isolation of glycans and/or polypeptides from body fluid samples for diagnostics, and quality control of glycosylation in engineered proteins, e.g., in therapeutic antibodies. |