Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56541df79a26070c6c2c585b54af47c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e71293e8c653f99fb08d7739f2f425f |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate |
2013-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83d0bbfd2f34b7f5a8b0221c6001da37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0dcd88a79fb14b25af4b13453bcd0b72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74f562b4e4fba9ec1b8c7a65e4794cb2 |
publicationDate |
2013-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2013191265-A1 |
titleOfInvention |
Method for producing protein-nucleic acid complex, and method for detecting target substance |
abstract |
Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gln) residue or a lysine (Lys) residue to the 3'-terminal of a nucleic acid using a 3'-terminal addition enzyme that can add a nucleotide to the 3'-terminal of a nucleic acid; and a labeling compound binding step of binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gln) residue using a transglutaminase (TGase) or binding a labeling compound having a glutamine (Gln) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase). |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11079372-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2015326827-B2 |
priorityDate |
2012-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |