http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013181265-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad9ac7e3526316d2f28b227959b33cc2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75cb9f951e941fb21d71cf8618641dab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_312ce60f92ba23d5a9c2b4bc10f31497 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36ec1612b81cdd14d1d42c180ec3826a |
publicationDate | 2013-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013181265-A1 |
titleOfInvention | Eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries |
abstract | Novel hyperactive Hermes Transposase mutants and genes encoding them are disclosed. These transposases are easily purified in large quantity after expression in bacteria. The modified Hermes Transposases are soluble and stable and exist as smaller active complexes compared to the native enzyme. The consensus target DNA recognition sequence is the same as the native enzyme and shows minimal insertional sequence bias. These properties are useful in whole genome sequencing applications that involve sample DNA preparation requiring simultaneous fragmentation and attachment of custom sequences to the ends of the fragments. Methods and compositions using these transposases in fragmentation and 5' end-tagging are also disclosed. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015157611-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11597974-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11519032-B1 |
priorityDate | 2012-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.