| abstract |
Bacteria are genetically engineered to produce 3-hyrdoxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/C0?2#191. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient. |