http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013143438-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f0cd67594fa53066a4c30aa36f9a2e4 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
filingDate | 2013-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5881ebc1db44054352d59916d300bcd2 |
publicationDate | 2013-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013143438-A1 |
titleOfInvention | Nucleic acid molecular cloning method based on homologous recombination, and related reagent kit |
abstract | The application provides a nucleic acid molecular cloning method based on homologous recombination. According to the method provided by the application, a target DNA is cloned to a vector through homologous recombination by providing a linearizing vector with two ends respectively added with a sequence (namely, a specific homologous arm of the target DNA) homologous with a sequence at two ends of the target DNA or a flanking sequence of the target DNA; or by utilizing a connecting section containing the specific homologous arm of the target DNA as well as the specific homologous arm of the vector (a sequence homologous with a specific region of the vector). The method provided by the application is especially applicable to the clone of a large DNA section and polymorphism researches of mononucleotide. The application further provides a related reagent kit. |
priorityDate | 2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.