| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2c077a4808c7e543df1e0524fa842ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_93a5d37b3fcc684325f7bf4a6c2708ce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6fbeae9a97963a1b03e73c4d34810be4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-735
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4726
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3828
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-775 |
| filingDate |
2013-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d37fe6b1bb5f2f79803189c9ead265c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a6e9aa3c09e32a93f552644fa6da12a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4bec1f31d87e0beac04fec4886bfa98 |
| publicationDate |
2013-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2013127816-A1 |
| titleOfInvention |
On-column enzymatic cleavage |
| abstract |
Herein is reported a method for obtaining a polypeptide by an immobilized metal ion affinity chromatography from a pro-polypeptide that comprise at its N- or C-terminus an metal ion affinity chromatography tag and a protease cleavage site comprising the step of recovering the polypeptide from the immobilized metal ion affinity chromatography column by incubating the bound pro-polypeptide with a protease, whereby the immobilized metal ion affinity chromatography material has been washed at least once with an urea solution. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017500040-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11360079-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11787868-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8791063-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11584793-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10370692-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11603411-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9139640-B2 |
| priorityDate |
2012-02-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |