http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013112358-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ef416fe4f66ebb1021cecfb0ba5ae45d |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
filingDate | 2013-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b351e75ff3d23519490e583197ef510f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2bd2c5a9488163a9ac3dc94c431846b |
publicationDate | 2013-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013112358-A1 |
titleOfInvention | Luciferase-linked methods for detecting adenosine and uses thereof |
abstract | Luciferase-linked methods for detecting adenosine and for screening for inhibitors of enzymes that produce adenosine are disclosed. The invention also provides methods for detecting the presence of Sadenosyl-L-homocysteine hydrolase (SAHH) comprising: (a) hydrolysis of S-adenosyl-Lhomocysteine (SAH) catalyzed by S-adenosyl-L-homocysteine hydrolase (SAHH) to adenosine and L-homocysteine; (b) converting adenosine to adenosine monophosphate (AMP) by adenosine kinase in the presence of a phosphate donor; (c) converting AMP to adenosine 5 '-triphosphate (ATP) by pyruvate phosphate dikinase (PPDK) in the presence of phosphoenolpyruvate (PEP) and inorganic pyrophosphate; and (d) reacting A TP with luciferin and Oz in the presence of luciferase to produce AMP and a luminescent signal, wherein the luminescent signal indicates the presence of S-adenosyl-L-homocysteine hydrolase (SAHH). |
priorityDate | 2012-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.