http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013110408-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ba165d54ce666317fde4c5799ce603e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2201-06113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2201-12 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-367 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B27-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-361 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B27-58 |
| filingDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77219e2cc431c77c84bd666013e14db3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7adf90a3eddb3edcbe944ef8cc5f5c1d |
| publicationDate | 2013-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2013110408-A1 |
| titleOfInvention | Microscope and method for high-resolution 3d fluorescence microscopy |
| abstract | A method for high-resolution 3D fluorescence microscopy, wherein fluorescence emitters in a sample (2) are repeatedly excited to emit fluorescence and individual images (10) are generated from the sample (2) using a microscope (1) that has an imaging beam path (4) having an optical resolution and a focal plane, wherein the fluorescence emitters are excited to emit fluorescence in such a way that at least some of the fluorescence emitters are isolated in each individual image (10) in such a way that the images (11) of these fluorescence emitters can be separated into individual images (10) during optical resolution; in the individual images (10) generated from the images of the isolated fluorescence emitters, the layers of these fluorescence emitters are localised with a location precision that exceeds the optical resolution and a high-resolution overall image is generated, each individual image being divided into a first and second partial image (12, 13) by means of a divider element (7), wherein the first partial image (12) represents a first focal plane of the sample (2) and the second partial image (13) represents a second focal plane in the sample (2), and both partial images (12, 13) are shown separately, characterised in that an adaptive mirror (7) is used as a divider element and is arranged substantially in a pupil of the imaging beam path (4), said adaptive mirror (7) being adjusted such that it shows two partial images (12, 13) temporally or locally separated, said adaptive mirror (7) being adjusted such that it provides two different focal lengths for the two partial images (12, 13). |
| priorityDate | 2012-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.