http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013081461-A3
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a42bad2e19a682b92b448e097c61b74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c684df17c1e09127a3765f1138203f85 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-538 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-743 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-538 |
filingDate | 2012-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f396f7c1aa95a43b6348a8e91ef066c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_227d0dc27b755e07f0c5b8345a22a98f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7860036114eeaa3cf6e7127aad283dc2 |
publicationDate | 2013-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013081461-A3 |
titleOfInvention | Methods and kits for determining protein-bound biomarkers |
abstract | The invention relates to the field of diagnostic methods, in particular to detection of biological molecules using mass spectroscopy (MS). Provided is an automated high-throughput method for quantitating a low molecular weight protein-bound biomarker directly in an isolated biological sample, comprising the steps of: (a) automated prepurification of the sample using an effective amount of an acid protease under conditions that allow for digestion of one or more binding proteins; (b) applying said protease-digested sample onto an on-line SPE column to capture at least part of said biomarker, followed by sequential washing of the solid phase; (c) eluting a fraction comprising said biomarker directly onto a liquid chromatography (LC) column comprising an apolar stationary phase and subjecting it to LC-MS or LC-MS-MS measurements to determine the amount of at least one biomarker; and (d) quantitating the biomarker(s). Also provided are kits for use in such method, for instance in the determination of vitamin D derivatives, testosterone and/or or melatonin. |
priorityDate | 2011-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.