| abstract |
The present invention provides a method for producing a cell belonging to an endodermal lineage, including inducing differentiation of a blood cell-derived iPS cell, specifically, a method for producing a cell belonging to an endodermal lineage, inducing the following steps (1) and (2): (1) a step of producing an iPS cell by contacting a nuclear reprogramming substance with a blood cell (2) a step of inducing differentiation of the iPS cell obtained in step (1) into a cell belonging to an endodermal lineage. In addition, the present invention provides a method for inducing differentiation of a human pluripotent stem cell into a CXCR4 positive endoderm cell, including (1) cultivating a human pluripotent stem cell in a single-cell state in the presence of activin A and Wnt3a for 4-10 days and (2) cultivating in the co-presence of 0.1-0.8 mM sodium butyrate for 0-10 days during the culture period. |