| abstract |
Proteases comprising an amino acid sequence that is at least 70% identical to the amino acid sequence given in SEQ ID NO: 1 over its total length, and that have R99E or R99D amino acid substitution in the numbering according to SEQ ID NO: 1 in combination with at least two additional amino acid substitutions selected from the group consisting of S3T, V4I and V199I, show a very good purification performance, especially for blood-containing fouling, and excellent temperature stability. |