http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013053859-A1

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filingDate 2012-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43d7030049120c05266e591f3061ea9c
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publicationDate 2013-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2013053859-A1
titleOfInvention High-resolution luminescence microscopy
abstract A microscopy method for producing a high-resolution image (55) of a sample (2), the method comprising the following steps: a) the sample (2) is furnished with a marker that emits statistically flashing luminescence radiation after excitation, or a sample (2) is used that has molecules that emit statistically flashing luminescence radiation after excitation, b) the sample (2) is excited to luminescence in such a manner that the marker/molecules emit luminescence radiation flashing at a flash rate, wherein - the illumination is structured in such a manner that the flash rate varies locally and - the sample (2) is repeatedly illuminated in different illumination states (.o1-.o9) of the structured illumination, so a different local distribution of the flash rate is obtained for each illumination state, c) the luminescing sample (2) is repeatedly imaged on a detector (6) in each of the different illumination states, so that an image sequence (44) is obtained for each of the different illumination states (.o1-.9), d) a raw image (40) reproducing the local distribution of the flash rate in the sample (2) is generated from each image sequence (44), e) the high-resolution image (43), which has a position resolution greater than that of the image on the detector (6), is generated from the obtained raw images (40.o1-40.o9) by computational processing (42) comprising Fourier filtering.
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