http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013053859-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ba165d54ce666317fde4c5799ce603e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06F3-0412 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-0076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-361 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B27-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-367 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B27-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-00 |
filingDate | 2012-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43d7030049120c05266e591f3061ea9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c7100a789aaf66b576f0214df8fe9fc |
publicationDate | 2013-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2013053859-A1 |
titleOfInvention | High-resolution luminescence microscopy |
abstract | A microscopy method for producing a high-resolution image (55) of a sample (2), the method comprising the following steps: a) the sample (2) is furnished with a marker that emits statistically flashing luminescence radiation after excitation, or a sample (2) is used that has molecules that emit statistically flashing luminescence radiation after excitation, b) the sample (2) is excited to luminescence in such a manner that the marker/molecules emit luminescence radiation flashing at a flash rate, wherein - the illumination is structured in such a manner that the flash rate varies locally and - the sample (2) is repeatedly illuminated in different illumination states (.o1-.o9) of the structured illumination, so a different local distribution of the flash rate is obtained for each illumination state, c) the luminescing sample (2) is repeatedly imaged on a detector (6) in each of the different illumination states, so that an image sequence (44) is obtained for each of the different illumination states (.o1-.9), d) a raw image (40) reproducing the local distribution of the flash rate in the sample (2) is generated from each image sequence (44), e) the high-resolution image (43), which has a position resolution greater than that of the image on the detector (6), is generated from the obtained raw images (40.o1-40.o9) by computational processing (42) comprising Fourier filtering. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2801854-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014180566-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9874737-B2 |
priorityDate | 2011-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.