http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013053852-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c57ad788d76c0d8b5cb98becd1405888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2cbadce7eeffaca3f0d28758cfe20a41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2278e9abdba35847e74df6e8c276a50 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b798c5e26a5f94cda84789141fee0f3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ed85a0b08a1b5c89edd3f7ccbf29834 |
| publicationDate | 2013-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2013053852-A1 |
| titleOfInvention | Design of stem-loop probes and utilization in snp genotyping |
| abstract | A stem-loop probe for single nucleotide polymorphism (SNP) genotyping of individual SNP nucleic acid target sequences comprises first (1), second (2), and third (3) single stranded nucleic acid portions. The second single stranded nucleic acid portion (2) is located between the first (1) and the third (3) single stranded nucleic acid portions. The first (1) and the third (3) single stranded nucleic acid portions build a double stranded, intramolecular stem (10). The second single stranded nucleic acid portion (2) forms a single stranded oligonucleotide loop (20) with a nucleotide sequence that is complementary to individual SNP nucleic acid target sequences. The nucleotide sequence of the stem-loop probe is chosen such that perfect match probe/target hybrids have a melting point Tm that is at least 5 °C higher than the T m of mismatched probe/target hybrids. The first (1) and the third (3) single stranded nucleic acid portions of the stem-loop probe comprise a 3' or 5' end configured as an A, T, or C nucleotide, to which A, T, or C nucleotide a non-quenched fluorophore is conjugated. In a method of detecting single nucleotide polymorphism (SNP) in nucleic acid containing samples, a pair of such stem-loop probes for SNP genotyping of two individual SNP nucleic acid target sequences of a sample is utilized. The stem-loop probes (100) comprise the same first (3), second (2), and third (3) single stranded nucleic acid portions and a ratio of perfect match probe/target hybrids to mismatched probe/target hybrids is detected at a certain temperature. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022232542-A3 |
| priorityDate | 2011-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.